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Specific tools for a new generation of sequencing Third Generation Sequencing (TGS) is getting a notable increase in relevance by surpassing some of the limitations of NGS technologies. In that sense, the main feature of TGS is the capacity to produce much longer reads than NGS. These long reads facilitate the assembly of complex genomes and the study of alternative

What is Monocle 3? Monocle 3 is a scRNA-Seq data analysis toolkit developed by Trapnell lab. It is written in R and introduced the notion of pseudotime. Monocle 3 can help perform three major types of scRNA-data analysis: Clustering & classification of cells by putative cell types Assignment of pseudotime to cells and, in turn, construction of single-cell trajectories to

Transcriptome analysis is a challenging bioinformatic problem. In eukaryotic transcriptomes, alternative splicing and alternative polyadenylation are the most complex issues. Genome-wide analysis of alternative splicing has been studied with short-read RNA sequencing for a while. Nevertheless, this technology needs a subsequent assembly, which is difficult to be resolved in ambiguities in complex loci.