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The OmicsBox Genome Analysis module allows to characterize and analyze newly sequenced genomes, from raw reads to gene structures in an efficient and user-friendly way.
Quality Control And Assessment
Use FastQC and Trimmomatic to perform the quality control of your samples, to filter reads and to remove low quality bases.
The assembly feature allows to reconstruct whole genome sequences without a reference genome or specific hardware requirements. Assemble sequencing data from both, short and long read technologies with 3 different algorithms: ABySS, SPAdes and Flye.
Alignment and Polishing
Align short sequencing reads against large sequences with BWA, and correct draft assemblies from long reads with Pilon.
Repeat Masking and Gene Finding
Mask repeats of genome assemblies with RepeatMasker to improve downstream gene predictions.
Perform prokaryotic (Glimmer) and eukaryotic (Augustus) gene predictions to characterize genome structure.
- DNA-Seq de novo assembly with ABySS 2
- DNA-Seq de novo assembly with SPAdes
- DNA-Seq de novo assembly with Flye
- DNA-Seq alignment with BWA
- DNA-Seq polishing with Pilon
- Repeat masking with RepeatMasker
- Eukaryotic gene finding with Augustus
- Prokaryotic gene finding with Glimmer