The OmicsBox Genome Analysis module allows to characterize and analyze newly sequenced genomes, from raw reads to gene structures in an efficient and user-friendly way.
Quality Control And Assessment
Use FastQC and Trimmomatic to perform the quality control of your samples, to filter reads and to remove low quality bases.
De-Novo Assembly
The assembly feature allows to reconstruct whole genome sequences without a reference genome or specific hardware requirements. Assemble sequencing data from several technologies with 2 different algorithms: ABySS and SPAdes.
Repeat Masking
Mask repeats and low complexity DNA sequences of your eukaryotic genome assemblies with RepeatMasker to improve downstream gene predictions.
Gene Finding
Perform prokaryotic (Glimmer) and eukaryotic (Augustus) gene predictions to characterize genome structure. The eukaryotic gene prediction offers RNA-seq intron hint support.
- DNA-Seq de novo assembly with ABySS 2
- DNA-Seq de novo assembly with SPAdes
- Repeat masking with RepeatMasker
- Eukaryotic gene finding with Augustus
- Prokaryotic gene finding with Glimmer
Genome Browser
Visualize your annotations in form of tracks to combine the genome sequences (.fasta) with alignments (.bam), intron-exon structure (.gff) and variant data (.vcf).
Statistics
Exploratory Analysis
The rich user interface allows to process large genome annotations with ease. Gene annotations, in General Feature Format style, can be filtered, sorted and combined with other result sets. Select the genes to display directly from the table.
Workflows
Eukaryotic Genome Analysis
Generate a genome draft of eukaryotic species by assembling DNA-Seq reads without additional prior information. Detect and mask repetitive sequences and improve the gene prediction by providing RNA-Seq data.
Prokaryotic Genome Analysis
Genomes of bacteria and other prokaryotic organisms can be assembled and characterized in a fast and sensitive way. Proceed with the functional annotation of the resulting gene sequences.
