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It is possible to run Blast only on the differential expressed genes and not on all the data with OmicsBox. One has to select only those sequences that have differential expressed genes in the OmicsBox project.First, make sure that the name of the sequences in the project match the ones from the differential expression results.
OmicsBox should be cited as follows: OmicsBox – Bioinformatics Made Easy, BioBam Bioinformatics, March 3, 2019, https://www.biobam.com/omicsbox In case you run functional annotation pipeline on your data (Mapping and Annotation), then the citation is as it used to be, Blast2GO.This is for the Blast2GO methodology: Götz S., Garcia-Gomez JM., Terol J., Williams TD., Nagaraj SH., Nueda MJ., Robles M., Talon
Create a taxonomic mapping file to Make Blast Database within OmicsBox OmicsBox allows creating a custom database to run Blast locally. The blast algorithm will run on the user’s computer against a database that is installed locally.In order to do so, we have to either download a pre-formatted NCBI database (see tutorial) or format our own database (see this tutorial
How to combine pairwise differential expression results with a functional annotation project in OmicsBox.
Once differential expression results are retrieved, it could be interesting to assign functional annotations to the differentially expressed genes and visualize the results in a single project. This is possible in OmicsBox.
This article explains how to export a GFF file with GO terms in Blast2GO.
This article explains how to start functional enrichment analysis of pairwise differential genes in Blast2GO.
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