Tips And Tricks

Mini-tutorials for common use-cases and to address frequently asked questions FAQs

Load Tair Gene Ids from Biomart

Load Sequences/ Annotation from a list of identifiers within Blast2GO

OmicsBox/Blast2GO offers two different features to retrieve the gene/protein sequences as well as the corresponding annotation from a list of identifiers within Blast2GO PRO. Both features can be found under File > Load > Load Annotations. The expected input file is a text file with the identifiers in a single column without a header.

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Load exon sequences from reference

Load FASTA sequences from Reference and GFF file

Sometimes databases provide the whole genome and the GFF or GTF files but not the exon or CDS FASTA files.With OmicsBox/Blast2GO it is possible to load a Fasta sequences and to extract the exons or the CDS from the genome using the GFF file.

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How to Change the Temp Folder Location for Blast2GO

OmicsBox/Blast2GO can handle big data, but it needs plenty of free disk space in the systems temporary files folder. If you run into troubles with a shortage of disk space, follow this guide to manually change the location of the folder for Blast2GO temporary files to e.g. another partition.

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How to Set Maximum Memory for Blast2GO

OmicsBox/Blast2GO automatically reserves up to 80% of the available system memory (i.e. RAM), which is fine in most cases. However, sometimes and especially on Linux, this can lead to excessive swapping and will slow down Blast2GO. Follow this guide to manually change the maximum amount of memory Blast2GO will be allowed to use.

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Some parts of Blast2GO 5 are not working (Show Blast Results, Welcome Part, Create Workflow) running Linux system.

You need to install some packages depending on your distribution in order to make some parts of Blast2GO to work. For Ubuntu/Debian: apt-get install libxss1 libgconf-2-4 For Fedora: yum install libXScrnSaver libXScrnSaver.i686 GConf2 For CentOS: yum install libXScrnSaver GConf2 For more information on Show Blast Results or create Workflows, please have a look at the OmicsBox user manual online.

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How to run Blast2GO installer in Linux systems?

To the Blast2GO installer in Linux systems follow the next steps: 0. Open a terminal in the folder where you have downloaded the installer. 1. Unzip the file: tar -xvf Blast2GO_unix_X_X_XX.zip 2. Make sure the .sh file has execution permissions: chmod +x Blast2GO_unix_X_X_XX.sh 3. Execute the .sh file: ./Blast2GO_unix_X_X_XX.sh An installation wizard should open. Just follow the instructions on the

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