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Tips, Tricks and Video Tutorial

Mini-tutorials for common use-cases and to address frequently asked questions FAQs

Combined Pathway Analysis with OmicsBox

Introduction A pathway analysis is an important step in the final biological interpretation of experimental results in most omics studies. For non-model species however this often becomes a difficult endeavor due to the lack of functional information. The Combined Pathway Analysis tool allows to identify pathways from multiple pathway databases for any set of sequences. In combination with differential expression

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New OmicsBox Login Dialog

With OmicsBox version 2.0 we introduce a new login dialog. This login is required for a more advanced subscription usage management. The login credentials are identical with the ones used for the BioBam Account website. If you do not yet have an BioBam Account you can easily create one from within OmicsBox via “SIGN UP”. If you forgot your password

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InterProScan with Blast2GO: How to make it work again

Activate development on Blast2GO was discontinued in 2018. All its functionality and more new features are now part of OmicsBox. Because Blast2GO is no longer updated, some of its functions may have stopped working because web services have changed or security certificates have expired, among other things. It is highly recommended to use OmicsBox instead of Blast2GO to benefit from

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Cloud Blast and Cloud Units: How to use resources wisely

This article describes how to run CloudBlast wisely. OmicsBox allows you to perform NCBI Blast searches using a cloud system for high-performance computing. This system runs jobs in parallel and autoscales depending on demand. To control usage we use so-called CloudUnits. These units represent computation time (cpu seconds) on the AWS infrastructure. At the moment only Blast and InterProScan consume

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How to run Blast of the differential expressed genes?

It is possible to run Blast only on the differential expressed genes and not on all the data with OmicsBox. One has to select only those sequences that have differential expressed genes in the OmicsBox project.First,  make sure that the name of the sequences in the project match the ones from the differential expression results.

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how to cite omicsbox

How to cite OmicsBox, Blast2GO and all available features

OmicsBox should be cited as follows: OmicsBox – Bioinformatics Made Easy, BioBam Bioinformatics, March 3, 2019, https://www.biobam.com/omicsbox In case you run functional annotation pipeline on your data (Mapping and Annotation), then the citation is as it used to be, Blast2GO.This is for the Blast2GO methodology: Götz S., Garcia-Gomez JM., Terol J., Williams TD., Nagaraj SH., Nueda MJ., Robles M., Talon

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Multilocus Sequence Typing MLST Analysis Tool

The Multilocus Sequence Typing Analysis Tool of OmicsBox (MLST) is a nucleotide sequence-based approach of characterizing isolates of bacterial species using the sequences of internal fragments of seven housekeeping genes. This video shows step-by-step how to analyze bacterial sequences with the MLST App in OmicsBox. As input, users can provide either DNA-Seq reads in FASTQ or contigs/scaffolds in FASTA format.

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Select .pal files to Run LocalBlast

How to create a taxonomic mapping file.

Create a taxonomic mapping file to Make Blast Database within OmicsBox OmicsBox allows creating a custom database to run Blast locally. The blast algorithm will run on the user’s computer against a database that is installed locally.In order to do so, we have to either download a pre-formatted NCBI database (see tutorial) or format our own database (see this tutorial

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