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Tips, Tricks and Video Tutorial

Mini-tutorials for common use-cases and to address frequently asked questions FAQs

Retrieve Top Blast Hit

How to change sequence names coming from Prokaryotic GeneFinding

When running GeneFinding the sequences receive a name with the predicted genes. The first part of the sequence identifier comes from the genome reference sequence name (de-novo assembly) and then a _orfx is appended, where x is a number. Sometimes this name is not useful to proceed with downstream analysis or compare results from other experiments. Is there any way

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Generate Gene Venn Diagram Representations with OmicsBox

What is a Gene Venn Diagram? A  Gene Venn Diagram is a graphical representation in the form of overlapping circles widely used to represent relationships among different genes (IDs).  High-throughput sequencing technologies generate large amounts of data sets coming from multiomics studies. At the transcriptomic level, differential expression analysis appears to be a highly important field of study in bioinformatics

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Load Tair Gene Ids from Biomart

Load Sequences/ Annotation from a list of identifiers within Blast2GO

OmicsBox/Blast2GO offers two different features to retrieve the gene/protein sequences as well as the corresponding annotation from a list of identifiers within Blast2GO PRO. Both features can be found under File > Load > Load Annotations. The expected input file is a text file with the identifiers in a single column without a header.

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Load exon sequences from reference

Load FASTA sequences from Reference and GFF file

Sometimes databases provide the whole genome and the GFF or GTF files but not the exon or CDS FASTA files.With OmicsBox/Blast2GO it is possible to load a Fasta sequences and to extract the exons or the CDS from the genome using the GFF file.

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