This tutorial shows how to assign subcellular localizations with PSORTb in OmicsBox/Blast2GO. We also explain how to merge the obtained results to the existing functional annotations of a Blast2GO project. OmicsBox/Blast2GO allows to assigns sub-cellular localization sites to proteins based on their amino acid sequence via PSORTb. PSORTb is an algorithm which can be applied to bacteria or archaea protein sequences
The Coloured Graph option is a function in Blast2GO which allows to combine and visualize multiple functional profiles within one GO graph. For each group or profile a different color can be assigned and the resulting GO graph can be filtered and colored accordingly. This video shows how to create a text files to generate a Coloured Graph for three
Some parts of the application are not working (Show Blast Results, Welcome Part, Create Workflow) running Linux system.
This applies to OmicsBox or Blast2GO 5. You need to install some packages depending on your distribution in order to make some parts work. For Ubuntu/Debian: apt-get install libxss1 libgconf-2-4 For Fedora: yum install libXScrnSaver libXScrnSaver.i686 GConf2 For CentOS: yum install libXScrnSaver GConf2 For more information on Show Blast Results or create Workflows, please have a look at the OmicsBox
Reformat/adapt Blast XML results against a custom UniProt sequence database to generate a species distribution chart Problem: Empty species distribution chart Solution: Reformat your SwissProt/UniProt blast XML results The species distribution chart is a good way to visualise the species found for all blast hits for a given dataset. It is possible to generate this chart with OmicsBox from the toolbar: functional
(Release Date: 3 November 2015) We released a new version (v.1.4.4) of our successful Blast2GO PRO Plugin for the CLC bio Genomics Workbench with new features and improvements. New features: Batch Rename your sequence names Set To Sense: Switch the sense of your sequences based on the best blast hit frame Longest ORF: Crop and convert sequences to longest ORF
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