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Use Cases, Reviews, Tutorials

Product Tutorial, Quickstarts, New Features, etc.

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Evaluation of Eukaryotic Gene Finding with Augustus in OmicsBox

Carlos Menor October 17, 2016

The OmicsBox Genome Analysis Module allows executing eukaryotic de-novo and RNA-seq based gene finding with Augustus. In this way, it is possible to discover novel, putative coding genes and their genomic positions for yet uncharacterized genome. Based on the Augustus algorithm an ‘ab-initio’ (DNA sequences only), as well as RNA-seq guided (BAM files) gene predictions, are supported. We will show

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Time Course Expression Analysis

Carlos Menor October 6, 2016

A simple use-case comparing OmicsBox with R chunks for Time Course Expression Analysis The Blast2GO feature “Time Course Expression Analysis” is designed to perform time-course expression analysis of count data arising from RNA-seq technology. Based on the software package ‘maSigPro’, which belongs to the Bioconductor project, this tool allows the detection of genomic features with significant temporal expression changes and

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Pairwise Differential Expression Analysis

Carlos Menor September 26, 2016

A simple use-case comparing OmicsBox with R chunks The OmicsBox feature “Pairwise Differential Expression Analysis” is designed to perform differential expression analysis of count data arising from RNA-seq technology. This tool allows the identification of differential expressed genes considering two different conditions based on the software package ‘edgeR’, which belongs to the Bioconductor project. This use case shows the basic

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Functional Analysis of Pancreatic Cancer Expression Profiles

Stefan September 8, 2016

This use case shows how to perform a functional analysis of pancreatic cancer expression data with Blast2GO. The performed steps are explained more in detail with short video tutorial linked to each section. Malignant (PDAC), benign (CP) and normal tissue (NP) gene lists are compared against each other. The human genome functional annotation data is retrieved via BioMart and used

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Brief review: Gene-Finding for Bacterial Genomes

Mariana Monteiro November 24, 2015

Introduction You have: Newly aligned genome of a bacterial non-model organism. You want: Perform functional annotation and analysis of its potential proteins. You need: Predict all potential genes or coding regions before proceeding to the functional annotation: Gene-Finding How can this be done? Use Glimmer, a set of algorithms which uses interpolated Markov models to distinguish coding from non-coding DNA in bacteria, archaea, and viruses. Glimmer

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Amazon AWS NCBI Blast Search

David Seide November 23, 2015

(This feature is now obsolete.) In the following article, I will explain how to set up an EC2 instance with the Blast+ AMI. This NCBI webpage takes us to the most recent Blast+ AMI in the AWS Marketplace, which we want to configure after hitting “Continue” on the right-hand side. We can now play around with the Region and EC2

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Command Line Examples

Stefan September 25, 2015

Blast2GO Command Line Examples (Version 1.4 release date: 01/10/2015) In the Blast2GO Command Line Manual (PDF) you can now find several practical examples which will help you to get started with Blast2GO over the command line. Request your trail here.

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