Reference-free analysis of long-read RNA sequencing for non-model species

  With improvements in accuracy and steadily decreasing costs, long-read sequencing technologies, such as the platforms provided by Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), have revolutionized the field of transcriptomics in recent years. Compared to short-read sequencing, which requires RNA fragmentation, long-read sequencing technologies can sequence RNA molecules in their entirety. This allows researchers to study the transcriptome

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Long-Reads Assembly with OmicsBox

Third-generation DNA sequencing technologies allows scientist to generate longer sequence reads, which can be used in whole-genome sequencing projects to yield better repeat resolution and more contiguous genome assemblies. However, although long-read sequencing technologies can produce genomes with long contiguity, the relatively high error rate of long reads has made it challenging to generate highly accurate final sequences. OmicsBox now

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Hybrid Genome Assembly in OmicsBox with SPAdes

DNA sequencing is the process of determining the nucleic acid sequence in DNA, and it is the technology by which the genome of a species can be characterized. Despite the advent of next-generation sequencing, current DNA sequencing technologies cannot read whole genomes at once, but rather reads small pieces of between 20 and 30.000 bases, depending on the technology used.

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