How to translate longest ORFs

How to translate longest ORFs

The Blast2GO “Translate Longest ORF” tool searches for the longest Open Reading Frame (ORF) in nucleotide sequences and translates them into their protein sequences. You may choose one or multiple of the six possible DNA frames or select the reading frame based on the frame of the best blastx hit. In this video, we will explain how to translate a set of Salmonella enterica genes and the available parameters (e.g how to select the reading frames, the genetic codes that can be used for translation, how to allow open-ended translations, etc). We also show the “Batch Rename” functions to undo sequence name changes.

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