How to use RFAM

How to use RFAM

Blast2GO allows searching for non-coding RNA (ncRNA) families and other structured RNA elements sequences via the RFAM webservice. The RFAM database is a collection of RNA families, categorized into three functional classes:

  • non-coding RNA genes
  • structured cis-regulatory elements
  • self-splicing RNAs

The RFAM algorithm allows via alignments to search this database and classify your sequence dataset. This might be interesting for contigs obtained via de-novo RNAseq, especially sequences with no similarity to any known proteins (no blast results – red sequences). 

The video shows how to run RFAM and interpret its results. Available summary charts are shown and explained.

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